BIBF0775

SKU: orb1302004

Description

BIBF0775 is a potent and selective inhibitor of the TGFβ type I receptor (ALK5), exhibiting an IC50 of 34 nM. This small molecule is a valuable research tool for studying TGF-β signaling in fibrosis, cancer, and other pathologies, with utility in both cellular and animal models.

Research Area

Cardiovascular Research, Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 334951-90-5
• MW: 494.63
• Purity: 99.79% (May vary between batches)
• Formula: C₃₁H₃₄N₄O₂
• SMILES: CCN(C)C(=O)c1ccc2\C(=C(\Nc3ccc(CN4CCCCC4)cc3)c3ccccc3)C(=O)Nc2c1
• Target: ALK,TGF-beta/Smad
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.04 mM);DMSO:11 mg/mL (22.24 mM)

Bioactivity

• Target IC50:
  ALK5:34 nM
• Cell Research:
  The high-content cytotoxicity assay (kit I) was performed according to the manufacturer's instructions. HaCaT cells were cultured overnight in black 96-well plates, incubated for 24 h with each compound at different concentrations, and stained with cytotoxicity cocktail. Cells were fixed, washed, and scanned on the Cellomics ArrayScan II platform. Images were analyzed with the Cell Health image analysis algorithm. Cytotoxicity indices were calculated for each of the four parameters (cellular membrane integrity, nuclear fragmentation and density, and lysosomal mass) to indicate the percentage of cells outside of the normal range which was defined using a vehicle-treated reference cell population.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BIBF 0775, BIBF0775, BIBF-0775, Alk5, inhibit, Inhibitor, Smad, Transforming growth factor beta receptors, TGF-b/Smad, TGFb, TGFbeta, TGFβR1(ALK5), TGF-β/Smad, TGF-β Receptor, TGFβ, TGFbeta/Smad, TGF-beta

Compound Identifiers

PubChem CID

135837779