BH3I-1

SKU: orb1308466

Description

BH3I-1

Research Area

Cell Biology, Protein Biochemistry

Key Properties

• CAS Number: 300817-68-9
• MW: 400.31
• Purity: 98.20%
• Formula: C₁₅H₁₄BrNO₃S₂
• SMILES: CC(C)C(N1C(=S)S\C(=C\c2ccc(Br)cc2)C1=O)C(O)=O
• Target: MDM-2/p53,E1/E2/E3 Enzyme,Bcl-2 Family
• Solubility: H2O:< 1 mg/mL (insoluble or slightly soluble);DMSO:60 mg/mL (149.88 mM);Ethanol:11 mg/mL (27.48 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (5 mM)

Bioactivity

• Target IC50:
  MDM2-p53:5.3 μM(Kd)|BH3-BCL XL:2.4 μM(Ki)
• In Vitro:
  BH3I-1 inhibits Bcl-xL heterodimerization in vitro. It also induces cytochrome c release. BH3I-1, while inhibiting its reported target Bcl-2/Bim and Bcl-xL/Bim, shows significant inhibition of both the p53/hDM2 and p300/Hif-1a interactions.
• Cell Research:
  Cells (5×104 cells per well) are seeded into white 96-well plates (Costar) and treated with various concentrations of the compounds for 48 h. For zVAD-FMK protection experiments, cells are preincubated with 100 μM zVAD-FMK for 1 h before the addition of chemicals. Cell viability is determined with an MTS assay. For PI staining experiments, cells are grown in 24-well plates and then incubated with 2 μg ml/L PI. Cell death is determined by FACS analysis.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

E1 Enzyme, E1 activating enzyme, E1Enzyme, E1/E2/E3 Enzyme, E3Enzyme, E2Enzyme, E2 Enzyme, E2 conjugating enzyme, E3 ligating enzyme, E3 Enzyme, Bcl-2 Family, Bcl-xL-BH3, BH 3I1, BH3I 1, BH3I1, BH-3I-1, BH3I-1, BHI 1, BHI1, BHI-1, MDM-2/p53, p53/MDM2, Inhibitor, inhibit, Ubiquitin activating enzyme, Ubiquitin conjugating enzyme, Ubiquitin ligase

Compound Identifiers

PubChem CID

6090108