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Description
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | E. coli |
|---|---|
| Target | Btc |
| Tag | N-6 His tag |
| MW | 12.2 kD |
| Purity | Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
| Protein Sequence | Asp32-Gln118 |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Lyophilized from a 0.2 um filtered solution of PBS,pH7.4. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Btc, Betacellulin, epidermal growth factor family member, isoform CRA_a, mCG_12529
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Human BTC Protein, hFc Tag [orb1743320]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 35.1 kDa after removal of the signal peptide. The apparent molecular mass of BTC-hFc is approximately 35-70 kDa due to glycosylation.
E.coli
10 μg, 50 μg, 100 μg Recombinant Mouse BTC Protein [orb3002291]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 12.2 KDa. Observed: 16 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human BTC Protein [orb3002519]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 34.5 KDa. Observed: 41-57 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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