Beta-Carotene

SKU: orb1224142

Description

β-Carotene is an organic compound and classified as a terpenoid. It is a precursor (inactive form) of vitamin A.(In Vitro):β-Carotene up-regulates PPAR-γ expression and ROS production in MCF-7 cancer cells.β-Carotene (1-100 μM; 72 hours) remarkably decreases the survival of MCF-7 cells in a dose-dependent manner.β-Carotene (50 μM; 24-72 hours) significantly enhances the expression levels of PPAR-γ mRNA and protein in a time-dependent manner.β-Carotene down-regulates the COX-2 but up-regulates the p21 mRNA level and protein expression in a time dependent manner.β-Carotene significantly increases the percentage of early apoptosis and the effect was partly attenuated by pre-incubation with GW9662 (HY-16578) or GSH (HY-D0187).β-Carotene induces cytochrome C release.

Key Properties

• CAS Number: 7235-40-7
• MW: 536.87
• Purity: >98% (HPLC)
• Formula: C₄₀H₅₆
• SMILES: CC(/C=C/C1=C(C)CCCC1(C)C)=C\C=C\C(C)=C\C=C\C=C(C)\C=C\C=C(C)\C=C\C2=C(C)CCCC2(C)C
• Target: Vitamin
• Solubility: Ethanol: 5 mg/mL warmed (9.31 mM); DMSO: 4 mg/mL warmed (7.45 mM)

Bioactivity

• In Vitro:
  β-Carotene up-regulates PPAR-γ expression and ROS production in MCF-7 cancer cells. β-Carotene (1-100 μM; 72 hours) remarkably decreases the survival of MCF-7 cells in a dose-dependent manner. β-Carotene (50 μM; 24-72 hours) significantly enhances the expression levels of PPAR-γ mRNA and protein in a time-dependent manner. β-Carotene down-regulates the COX-2 but up-regulates the p21 mRNA level and protein expression in a time dependent manner. β-Carotene significantly increases the percentage of early apoptosis and the effect was partly attenuated by pre-incubation with GW9662 (HY-16578) or GSH (HY-D0187). β-Carotene induces cytochrome C release. Cell Viability Assay Cell line: MCF-7 cells. Concentration: 1 μM, 10 μM, 20 μM, 50 μM, 100 μM. Incubation time: 72 hours. Result: Decreased the numbers of viable cells to 70% and 50% at 20 μMand 50 μM, respectively. RT-PCR Cell line: MCF-7 cells. Concentration: 50 μM. Incubation time: 24 hours, 48 hours, 72 hours. Result: Up-regulated the PPAR-γ mRNA. Western blot analysis. Cell line: MCF-7 cells. Concentration: 50 μM. Incubation time: 24 hours, 48 hours, 72 hours. Result: Up-regulated PPAR-γ protein expression levels. Apoptosis Analysis Cell line: MCF-7 cells. Concentration: 50 μM. Incubation time: 72 hours. Result: Induced MCF-7 cells apoptosis.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Food Orange 5 | KPMK | Lucarotin | NSC 62794 | Provatene | Provitamin A | Solatene

Quick Database Links

Gene Symbol

Vitamin