Bergapten

SKU: orb1306366

Description

Bergapten (5-Methoxypsoralen) is a furanocoumarin compound that acts as a cell proliferation inhibitor. It is widely used in biochemical research, particularly in studies of cell cycle arrest, photochemotherapy (PUVA) for skin disorders, and investigating DNA intercalation mechanisms in both cellular and animal models.

Research Area

Cell Biology, Metabolism Research

Key Properties

• CAS Number: 484-20-8
• MW: 216.19
• Purity: 99.95% (May vary between batches)
• Formula: C₁₂H₈O₄
• SMILES: COC1=C2C=CC(=O)OC2=CC2=C1C=CO2
• Target: Autophagy,Cytochromes P450
• Solubility: Ethanol:< 1 mg/mL (insoluble or slightly soluble);DMSO:13.82 mg/mL (63.93 mM);10% DMSO+90% Corn Oil:1 mg/mL (4.63 mM);H2O:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• In Vivo:
  In both tamoxifen-sensitive and -resistant breast cancer cells, Bergapten eliminates ERα and reprograms membrane signaling targeted at ERα, thereby enhancing the potential for cell mitosis.
• In Vitro:
  In C57BL/6J mice induced with acetaminophen (APAP), Bergapten exhibits antioxidant activity and mitigates hepatotoxicity.
• Cell Research:
  5-Methoxypsoralen (5-MOP) is dissolved in DMSO and stored, and then diluted with appropriate medium (final DMSO <0.1%) before use. The human colon adenocarcinoma cell line (COLO 205, from a 70-year-old male Caucasian) sre placed into 75-cm2 tissue culture flasks and grown in RPMI1640 medium, supplemented with 10% fetal bovine serum, containing penicillin and streptomycin (100 Ig/mL) and 1 mM glutamine, at 37°C in a humidified atmosphere of 5% CO2 and 95% O2. The human stomach adenocarcinoma cell line are placed into 75-cm2 tissue culture flasks and grown in RPMI 1640 medium, supplemented with 10% fetal bovine serum, containing penicillin and streptomycin (100 Ig/mL) and 1 mM glutamine, at 37°C in a humidified atmosphere of 5% CO2 and 95% O2. SC-M1 and COLO 205 cells are treated with different concentrations of 5-MOP (0.05, 0.5, 5, 10, 25 and 50 mM) and incubated for 72 h for the dose-effect study of 5-MOP on NAT activity. To determine the time-course effect of 0.5 mM 5-MOP on NAT activity, the cells are incubated at 37AC and harvested at 12, 24, 48 and 72 h, respectively. 5-MOP is dissolved in DMSO and the final concentration of vehicle is <0.1%. Only DMSO (solvent) is added for the control regimen.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CYPs, CYP, Cytochrome P450, Bergapten, 5-Methoxypsoralen, Autophagy, inhibit, Inhibitor

Compound Identifiers

PubChem CID

2355