BCI-121

SKU: orb1301890

Description

BCI-121 is a substrate-competitive inhibitor targeting the lysine methyltransferase SMYD3. It has been shown to suppress cancer cell proliferation in vitro and demonstrates potential for oncology research, including studies on tumor growth in vivo.

Research Area

Epigenetics & Chromatin

Key Properties

• CAS Number: 432529-82-3
• MW: 340.22
• Purity: 99.67% (May vary between batches)
• Formula: C₁₄H₁₈BrN₃O₂
• SMILES: NC(=O)C1CCN(CC(=O)Nc2ccc(Br)cc2)CC1
• Target: Histone Methyltransferase
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:4 mg/mL (11.76 mM);DMSO:100 mg/mL (293.93 mM)

Bioactivity

• In Vitro:
  BCI-121 significantly reduced the proliferation of HT29 (by 46%) and HCT116 (by 54%) cells at 72 h and decreased the expression levels of SMYD3 target genes. BCI-121 treatment only affected proliferation of cancer cell lines expressing high levels of SMYD3. The high levels of SMYD3 expression detected in OVCAR-3 cells, along with the strong reduction in proliferation rate observed upon BCI-121 treatment.
• Cell Research:
  Cell proliferation is determined using the cell proliferation reagent WST-1. Cells are seeded into 96-well plates one day before treatment. After 48 h, 72 h, or 96 h of BCI-121 or DMSO exposure, 10 μL of the Cell Proliferation Reagent WST-1 are added to each well and incubated at 37 °C in a humidified incubator for up to 1 h. Absorbance is measured on a microplate reader at 450/655 nm. The proliferation index is calculated as the ratio of WST-1 absorbance of treated cells to WST-1 absorbance of control cells.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BCI 121, BCI121, BCI-121, Inhibitor, HistoneMethyltransferase, Histone Methyltransferase, inhibit, SMYD3

Compound Identifiers

PubChem CID

795875