BCECF

SKU: orb1706388

Description

BCECF is a fluorescent small molecule dye widely used as a cytosolic pH indicator in both in vitro and in vivo research. Its sensitivity allows for real-time monitoring of intracellular pH dynamics in fields such as cell biology, physiology, and oncology.

Key Properties

• CAS Number: 85138-49-4
• MW: 520.44
• Formula: C₅₄H₄₀O₂₂
• SMILES: O=C(O1)C2=C(C=C(C(O)=O)C=C2)C31C4=C(C=C(O)C(CCC(O)=O)=C4)OC5=CC(O)=C(CCC(O)=O)C=C53 O=C(O1)C2=C(C=CC(C(O)=O)=C2)C31C4=C(C=C(O)C(CCC(O)=O)=C4)OC5=CC(O)=C(CCC(O)=O)C=C53
• Target: Others
• Solubility: H2O:< 0.1 mg/mL (insoluble);10% DMSO+40% PEG300+5% Tween-80+45% Saline:2.5 mg/mL (4.8 mM);DMSO:50 mg/mL (96.07 mM)

Bioactivity

• In Vitro:
  Methods: 1. 2-20mM BCECF mother liquor was prepared in anhydrous DMSO. 2. Use Hanks or PBS to prepare 5-50µM BCECF dye. 3. Add the configured BCECF dye to the cell culture plate. 1000 μL/well (6-well plate),100 μL/well (96-well plate) or 25 μL/well (384-well plate). 4, Use a fluorescence microscope or fluorescent plate reader to measure at Ex/Em = 490/535 nm or 430/535 nm. Results: The new use of BCECF as a fluorescent marker for hydrogen bodies (the first such marker) was demonstrated.

Storage & Handling

• Storage: keep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein, 2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein, BCECF

Compound Identifiers

PubChem CID

25069080