BAZ2B Antibody

SKU: orb1247738

Description

BAZ2B Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments gave an approx 35kDa band in Testis and Brain lysates after 0.3µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 221kDa according to NP_038478. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Small Intestine shows primarily nuclear staining in a number of cells of the lamina propria. Recommended concentration, 1-2µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-YFEKKWTDTFKVS
• Target: BAZ2B
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BAZ2B, bromodomain adjacent to zinc finger domain, 2B, DKFZP434H071, DKFZp762I0516, FLJ45644, WALp4

orb1247738 (5 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

BAZ2B

UniProt

Q9UIF8

RefSeq (Protein):NP_038478.2