BAY 87-2243

SKU: orb1305409

Description

BAY 87-2243 is a small molecule research compound that selectively and potently inhibits hypoxia-inducible factor-1 (HIF-1). It is widely used in both in vitro and in vivo studies to investigate tumor hypoxia, angiogenesis, and cancer metabolism.

Research Area

Cardiovascular Research, Cell Biology, Epigenetics & Chromatin, Metabolism Research

Key Properties

• CAS Number: 1227158-85-1
• MW: 525.53
• Purity: 99.95% (May vary between batches)
• Formula: C₂₆H₂₆F₃N₇O₂
• SMILES: Cc1cc(nn1Cc1ccnc(c1)N1CCN(CC1)C1CC1)-c1nc(no1)-c1ccc(OC(F)(F)F)cc1
• Target: Ferroptosis,HIF/HIF Prolyl-Hydroxylase,HIF
• Solubility: DMSO:9.2 mg/mL (17.51 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (3.81 mM)

Bioactivity

• In Vivo:
  In hypoxic lung cancer H460 cells, BAY 87-2243 inhibits the expression of HIF target genes and suppresses the accumulation of HIF-1α protein. It impairs cell proliferation by interfering with mitochondrial function under glucose consumption. Additionally, BAY 87-2243 inhibits the reporter gene activity of HIF-1 and the expression of CA9 protein, with IC50 values of 0.7 nM and 2 nM, respectively.
• In Vitro:
  BAY87-2243 (administered orally at 4 mg/kg) reduced tumor weight, HIF-1α protein, and HIF-1 target gene expression levels in an H460 xenograft mouse model.
• Cell Research:
  To evaluate the cytotoxicity of BAY 87-2243, 2.000 cells of the respective cell lines are seeded in 96-well plates and cultured in the appropriate growth medium containing 10% FCS. BAY 87-2243 at various concentrations is added at 24 h after seeding for additional 48 h and cell viability is determined using Cell Titer Glow Assay.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BAY 87 2243, BAY 872243, BAY 87-2243, inhibit, HIF-1α, HIF-PH, HIFs, HIFProlylHydroxylase, HIF/HIF ProlylHydroxylase, HIF Prolyl-Hydroxylase, HIF ProlylHydroxylase, HIF/HIF Prolyl-Hydroxylase, HIF/HIFProlylHydroxylase, HIF, Inhibitor, Ferroptosis, Hypoxia-inducible factors

Compound Identifiers

PubChem CID

67377767