BAM7

SKU: orb1305464

Description

BAM 7 is a small molecule that directly and selectively activates the pro-apoptotic protein Bax with an EC50 of 3.3 µM. It serves as a valuable research tool for studying mitochondrial apoptosis pathways in both cellular and biochemical assays, facilitating investigations into cancer biology and therapeutic strategies targeting Bcl-2 family proteins.

Research Area

Cell Biology

Key Properties

• CAS Number: 331244-89-4
• MW: 405.47
• Purity: 99.23% (May vary between batches)
• Formula: C₂₁H₁₉N₅O₂S
• SMILES: CCOc1ccccc1N\N=C1/C(C)=NN(C1=O)c1nc(cs1)-c1ccccc1
• Target: Bcl-2 Family
• Solubility: DMSO:4.1 mg/mL (10.11 mM)

Bioactivity

• Target IC50:
  Bax:3.3 mM(EC50)
• In Vivo:
  In vitro experiments show that BAM7 induces BAX-mediated pore formation, BAX oligomerization, and BAX-dependent cell death. By activating BAX within cells, BAM7 selectively triggers BAX-mediated apoptosis. Its efficacy is limited to cells containing BAX, where it induces the biochemical and morphological hallmarks of BAX-mediated apoptosis. BAM7 binds directly to the N-terminal BH3 domain of BAX, facilitating interaction at the surface and BAX activation via the BIM BH3 helix. Moreover, BAM7 dose-dependently and temporally triggers the conversion of BAX from monomers to oligomers.
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• Cell Research:
  MEFs (2.5 × 103 cells per well) are seeded in 96-well opaque plates for 18-24 h and then incubated with serial dilutions of BAM7, ANA-BAM16 or vehicle (0.15% (v/v) DMSO) in DMEM at 37 °C in a final volume of 100 μL. Cell viability is assayed at 24 h by addition of CellTiter-Glo reagent according to the manufacturer's protocol, and luminescence is measured using a SpectraMax M5 microplate reader. Viability assays are performed in at least triplicate, and the data are normalized to vehicle-treated control wells.(Only for Reference)
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, inhibit, Bcl-2 Family, Bax, BAM 7, BAM7, BAM-7

Compound Identifiers

PubChem CID

3101542