Bacopaside II

SKU: orb1297452

Description

Bacopaside II is a research compound with demonstrated anti-angiogenic properties. It inhibits endothelial cell migration and tube formation while promoting apoptosis, making it a useful tool for studying angiogenesis in cancer and vascular disease research, both in vitro and in vivo.

Research Area

Cell Biology, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 382146-66-9
• MW: 929.10
• Purity: 99.86% (May vary between batches)
• Formula: C₄₇H₇₆O₁₈
• SMILES: CC(C)=C[C@@H]1CO[C@]23C[C@]4(CO2)[C@H](CC[C@@H]2[C@@]5(C)CC[C@H](O[C@@H]6O[C@H](CO)[C@@H](O)[C@H](O[C@@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@H]7O)[C@H]6O[C@@H]6O[C@@H](CO)[C@H](O)[C@H]6O)C(C)(C)[C@@H]5CC[C@@]42C)[C@H]3[C@@]1(C)O
• Target: Apoptosis,Aquaporin
• Solubility: 10% DMSO+90% Corn Oil:1.00 mg/mL (1.08 mM);DMSO:10 mM

Bioactivity

• In Vitro:
  We tested the AQP1 inhibitor, Bacopaside II, derived from medicinal plant Bacopa monnieri, on endothelial cell migration and tube-formation in vitro using mouse endothelial cell lines (2H11 and 3B11) and human umbilical vein endothelial cells (HUVEC). The effect of Bacopaside II on viability, apoptosis, migration and tubulogenesis was assessed by a proliferation assay, annexin-V/propidium iodide flow cytometry, the scratch wound assay and endothelial tube-formation, respectively. Cell viability was reduced significantly for 2H11 at 15 μM (p = 0.037), 3B11 at 12.5 μM (p = 0.017) and HUVEC at 10 μM (p < 0.0001). At 15 μM, the reduced viability was accompanied by an increase in apoptosis of 38%, 50% and 32% for 2H11, 3B11 and HUVEC, respectively. Bacopaside II at ≥10 μM significantly reduced migration of 2H11 (p = 0.0002) and 3B11 (p = 0.034). HUVECs were most sensitive with a significant reduction at ≥7.5 μM (p = 0.037). Tube-formation was reduced with a 15 μM dose for all cell lines and 10 μM for 3B11 (p < 0.0001).

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, Inhibitor, Apoptosis, ATPase, Bacopaside II, P-gp

Compound Identifiers

PubChem CID

9876264