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Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK (Dpn) RR-NH2. The specific activity is > 1. 5 pmoles /min /μg. |
| Target | BACE1 |
| Tag | N-Fc Tag |
| MW | 75 kDa |
| Purity | >85% as determined by SDS-PAGE. |
| Protein Sequence | Thr 22 - Thr 457 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 50 mM tris, 100 mM glycine, pH 7.5 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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The purity of rh BACE1 Fc Chimera was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_036236.1 |
|---|
Documents Download
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Protocol Information
BACE1 Recombinant Protein (orb1228175)
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