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Catalog Number | orb1228412 |
---|---|
Category | Proteins |
Description | B7-1 Recombinant Protein |
Tag | C-6 His tag |
Form/Appearance | Lyophilized |
Purity | Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
MW | 24.7 kD |
Target | CD80 |
UniProt ID | P33681 |
Protein Sequence | Val35-Asn242 |
Source | Human Cells |
NCBI | P33681 |
Storage | Lyophilized protein should be stored at -20˚°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7˚°C for 2-7 days.Aliquots of reconstituted samples are stable at -20˚°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 um filtered solution of PBS, pH 7.4. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. |
Alternative names | CD80, Activation B7-1 antigen, B7, BB1, CD28LG1, C Read more... |
Note | For research use only |
Application notes | This recombinant protein can be used for biological assays. For research use only. |
Expiration Date | 6 months from date of receipt. |
ELISA, WB | |
> 95% as determined by SDS-PAGE. | |
50.9 kDa | |
HEK293 cells |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 50.9 kDa after removal of the signal peptide. The apparent molecular mass of B7-1-mFc-His is approximately 70-130 kDa due to glycosylation. | |
E.coli |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 50.0 kDa after removal of the signal peptide. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 49.8 kDa after removal of the signal peptide. The apparent molecular mass of mB7-1-hFc is approximately 70-100 kDa due to glycosylation. | |
Mammalian |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |