AVD Antibody

SKU: orb344845

Description

Avidin antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:2,000 - 1:20,000, IHC: 1:1,000 - 1:5,000, WB: 1:1,000 - 1:10,000
• Reactivity: Bacteria
• Application Notes:
  Anti-Avidin has been tested by western blot and is suitable for ELISA and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Avidin (Hen Egg White)
• Target: AVD
• Purity: Anti-Avidin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Avidin [Hen Egg White]. No cross reactivity occurs against Streptavidin.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Avidin Antibody, rabbit avidin egg white antibody, rabbit Anti Avidin

Western blot of Rabbit Anti-Avidin Antibody. Lane 1: Molecular Weight Marker. Lane 2: Avidin. Lane 3: Streptavidin. Primary Antibody: Anti-Avidin at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n orb347654) at 1: 40000 for 30 min at RT. Block: 0.75% Casein/TBS for 30 min. Expect MW: ~15.8 kDa. Exposure: 8 secs.

Quick Database Links

Gene Symbol

AVD

UniProt

P02701