AVD Antibody

SKU: orb344844

This product can be custom made

Description

Avidin antibody

Images & Validation

−

Item 1 of 1

Tested Applications	ELISA, IHC, WB
Dilution Range	ELISA: 1:2,000 - 1:20,000, IHC: 1:1,000 - 1:5,000, WB: 1:1,000 - 1:10,000
Reactivity	Bacteria
Application Notes	Anti-Avidin has been tested by western blot and is suitable for ELISA and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Key Properties

−

Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Avidin (Hen Egg White)
Target	AVD
Purity	Anti-Avidin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Avidin [Hen Egg White]. No cross reactivity occurs against Streptavidin.
Conjugation	Unconjugated

Storage & Handling

−

Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

rabbit anti-Avidin Antibody, rabbit avidin egg white antibody, rabbit Anti Avidin

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Western blot of Rabbit Anti-Avidin Antibody. Lane 1: Molecular Weight Marker. Lane 2: Avidin. Lane 3: Streptavidin. Primary Antibody: Anti-Avidin at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n orb347654) at 1: 40000 for 30 min at RT. Block: 0.75% Casein/TBS for 30 min. Expect MW: ~15.8 kDa. Exposure: 8 secs.