Auranofin

SKU: orb1309052

Description

Auranofin is a thioredoxin reductase (TrxR) inhibitor with an IC50 of 0.2 µM. This antirheumatic agent, used for rheumatoid arthritis, has demonstrated antiviral activity against SARS-CoV-2 in vitro and has been investigated in various cancer and infectious disease research models.

Research Area

Cell Biology, Neuroscience, Protein Biochemistry

Key Properties

• CAS Number: 34031-32-8
• MW: 678.49
• Purity: >98%
• Formula: C₂₀H₃₄AuO₉PS
• SMILES: O(C(C)=O)[C@@H]1[C@@H](OC(C)=O)[C@H]([S-][Au+][P](CC)(CC)CC)O[C@H](COC(C)=O)[C@H]1OC(C)=O
• Target: Kinase
• Solubility: Soluble in DMSO (up to 5 mg/ml) or in Ethanol (up to 4 mg/ml).

Bioactivity

• Target IC50:
  HPF cells:3 µM|A549 cells:5 µM|SK-LU-1 cells:5 µM|NCI-H1299 cells:1 µM|NCI-H4 cells60 cells:4 µM|TrxR:0.2 μM|Calu-6 cells:3 µM
• In Vivo:
  Prophylactic treatment of adjuvant-induced arthritis rats with auranofin results in a slight reduction in paw edema, a complete normalization of the depressed IL-2 production, and a reduction of the elevated IL-1 production, without effect on the depressed IL-3 production.
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• In Vitro:
  Auranofin, an established rheumatoid arthritis treatment, also shows promise across a spectrum of other conditions such as cancer and neurodegenerative diseases. It triggers apoptosis via a Bax/Bak-dependent pathway by selectively disrupting mitochondrial redox balance and oxidizing Prx3. Furthermore, auranofin hampers SKOV3 cell proliferation and survival in a dose- and time-specific manner, instigating caspase-3 activation, elevating pro-apoptotic Bax and Bim proteins, and diminishing the anti-apoptotic Bcl-2 in SKOV3 cells. This lipophilic gold-based agent, known for its anti-inflammatory and immunosuppressive characteristics, notably impedes cell growth and induces mitochondrial apoptosis in PC3 human prostate cancer cells, with a noteworthy reduction in cell viability at an IC50 of 2.5 µM after 24 hours.
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• Cell Research:
  Auranofin is dissolved in DMSO. Cells are treated with auranofin (0, 50, 100, 200 and 400 nM) for 72 h for the dose-dependent response assay and 100 nM of auranofin is added into the wells for 0, 24, 72 and 120 h for the time-dependent response assay. Control cultures are treated with DMSO. Cell viability is measured by the MTT assay.
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Storage & Handling

• Storage: RT
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SKF-39162

Quick Database Links

Gene Symbol

Kinase