ATP

SKU: orb1306758

Description

Adenosine triphosphate (ATP) serves as the primary energy currency within cells and also functions as an extracellular signaling molecule by activating purinergic receptors. It is widely used in research areas such as neurotransmission, immunology, and bone biology for both in vitro assays and in vivo studies.

Research Area

Metabolism Research

Key Properties

• CAS Number: 56-65-5
• MW: 507.18
• Purity: >99.99% (May vary between batches)
• Formula: C₁₀H₁₆N₅O₁₃P₃
• SMILES: O[C@H]1[C@H](N2C=3C(N=C2)=C(N)N=CN3)O[C@H](COP(OP(OP(=O)(O)O)(=O)O)(=O)O)[C@H]1O
• Target: Endogenous Metabolite
• Solubility: H2O:118.8 mg/mL (234.24 mM);DMSO:1.67 mg/mL (3.29 mM)

Bioactivity

• In Vivo:
  METHODS: To test the antibacterial activity in vivo, ATP (50 mg/kg) was injected intraperitoneally into Kunming mice, and E. coli 25922 or S. aureus 25923 was injected 1-24 h later. RESULTS: The administration of ATP 4 h or 24 h before the attack did significantly increase the survival rate of infected mice, regardless of the bacterial type. METHODS: To assay in vivo antibacterial activity, ATP (40 mg/kg), clarithromycin (12 mg/kg), and rifampin (8 mg/kg) were injected subcutaneously into MAC-infected BALB/c mice five times per week for eight weeks. RESULTS: Co-administration of ATP with clarithromycin and rifampin accelerated bacterial clearance in MAC-infected mice without resulting in changes in histopathologic features or mRNA expression of pro- or anti-inflammatory cytokines in mice not given ATP.
• In Vitro:
  METHODS: Synovial fibroblast HSF were rapidly treated (wash-in and wash-out ~2 s) with ATP (10 μM) three times, and [Ca2+] changes were detected using Fluo-4 fluorescence. RESULTS: During the first application of ATP, Fluo-4 fluorescence increased rapidly after a delay of a few seconds and decreased slightly before the end of ATP application. After removal of ATP, the fluorescence signal returned to resting levels, but fluorescence decreased much more slowly than it had begun after the initial ATP application. The two subsequent ATP applications produced a response of less amplitude than the first, and the delay until the onset of the response appeared to lengthen with successive applications. METHODS: Mouse bone marrow-derived macrophages BMDM were stimulated with LPS, HKEC, or HKSA, followed by treatment with ATP (2 mM) for 0.5-24 h. Levels of IL-1β, KC, and MIP-2 were determined using ELISA. RESULTS: ATP treatment strongly induced the secretion of IL-1β, KC and MIP-2.

Storage & Handling

• Storage: keep away from moisture,keep away from direct sunlight,store at low temperature,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Adenosine triphosphate, Adenosine 5'-triphosphate, Ara-ATP, Atipi, EndogenousMetabolite, Endogenous Metabolite, Triphosphaden, Triphosphoric acid adenosine ester

Compound Identifiers

PubChem CID

5957