Atglistatin

SKU: orb1307284

Description

Atglistatin is a potent and selective competitive inhibitor of ATGL, exhibiting an IC50 of approximately 0.7 µM for lipolysis suppression in vitro and demonstrating no toxicity up to 50 µM. This compound is a valuable research tool for studying lipid metabolism in cellular and animal models of metabolic disorders.

Research Area

Metabolism Research

Key Properties

• CAS Number: 1469924-27-3
• MW: 283.37
• Purity: 99.81%
• Formula: C₁₇H₂₁N₃O
• SMILES: CN(C)C(=O)Nc1cccc(c1)-c1ccc(cc1)N(C)C
• Target: Lipase
• Solubility: Ethanol:4 mg/mL (14.12 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:4 mg/mL (14.12 mM);DMSO:100 mg/mL (352.9 mM);H2O:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  ATGL:0.7 μM
• In Vivo:
  Atglistatin, administered orally (p.o.), reduces fatty acids (FA) and glycerol by up to 50% and 62% respectively in a dose-dependent manner, and also significantly reduces triglyceride (TG) levels in the plasma by 43%. When administered via intraperitoneal injection (i.p.), Atglistatin inhibits lipolysis in a dose- and time-dependent manner. Moreover, Atglistatin exhibits significant differences in tissue distribution, primarily accumulating in the liver and adipose tissue.
• In Vitro:
  Atglistatin inhibits ATGL, consequently suppressing lipid breakdown In both cellular and organ cultures without exhibiting cytotoxic effects at concentration up to 50 µM.
• Cell Research:
  For MTT-based in vitro viability assays, cells are seeded in 96-well plates and cultured under s andard conditions for 24 h the Next day, cells are pretreated with different concentration of Atglistatin dissolved in DMSO or Cisplatin dissolved in DMF as positive control for 2 h. Medium is replaced by an identical fresh medium and incubated again fo the indicated time points. Thereafter, cells are incubated for 3 h with 100 μL Thiazolyl Blue Tetrazolium Bromide (MTT) the resulting violet formazan crystals are dissolved by adding 100 μL of MTT solubilization solution (0.1% NP-40, 4 mM HCl and anhydrous isopropanol). After complete dissolution o the formazan product, absorbance is measured at 595 nM using 690 nM as reference wavelength.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

Inhibitor, inhibit, Adipose triglyceride lipase, ATGL, Atglistatin

Compound Identifiers

PubChem CID

71699712