Atglistatin

SKU: orb1307284

Description

Atglistatin is a potent and selective competitive inhibitor of ATGL, exhibiting an IC50 of approximately 0.7 µM for lipolysis suppression in vitro and demonstrating no toxicity up to 50 µM. This compound is a valuable research tool for studying lipid metabolism in cellular and animal models of metabolic disorders.

Research Area

Metabolism Research

Key Properties

• CAS Number: 1469924-27-3
• MW: 283.37
• Purity: >98%
• Formula: C₁₇H₂₁N₃O
• SMILES: CN(C)C(=O)Nc1cccc(c1)-c1ccc(cc1)N(C)C
• Target: Lipase
• Solubility: Soluble in DMSO (up to 5 mg/ml) or in Ethanol (up to 5 mg/ml with warming)

Bioactivity

• Target IC50:
  ATGL:0.7 μM
• In Vivo:
  Atglistatin, administered orally (p.o.), reduces fatty acids (FA) and glycerol by up to 50% and 62% respectively, in a dose-dependent manner, and also significantly reduces triglyceride (TG) levels in the plasma by 43%. When administered via intraperitoneal injection (i.p.), Atglistatin inhibits lipolysis in a dose- and time-dependent manner. Moreover, Atglistatin exhibits significant differences in tissue distribution, primarily accumulating in the liver and adipose tissue.
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• In Vitro:
  Atglistatin inhibits ATGL, consequently suppressing lipid breakdown in both cellular and organ cultures without exhibiting cytotoxic effects at concentrations up to 50 µM.
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• Cell Research:
  For MTT-based in vitro viability assays, cells are seeded in 96-well plates and cultured under standard conditions for 24 h. The next day, cells are pretreated with different concentrations of Atglistatin dissolved in DMSO or Cisplatin dissolved in DMF as positive control for 2 h. Medium is replaced by an identical fresh medium and incubated again for the indicated time points. Thereafter, cells are incubated for 3 h with 100 μL Thiazolyl Blue Tetrazolium Bromide (MTT). The resulting violet formazan crystals are dissolved by adding 100 μL of MTT solubilization solution (0.1% NP-40, 4 mM HCl and anhydrous isopropanol). After complete dissolution of the formazan product, absorbance is measured at 595 nm using 690 nm as reference wavelength.(Only for Reference)
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, inhibit, Adipose triglyceride lipase, ATGL, Atglistatin

Compound Identifiers

PubChem CID

71699712