ATG16L Antibody

• Catalog Number: orb1933395
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB07490
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Dilution range: IF: 1:25, WB: 1:1000, WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 68265 Da
• Target: This ATG16L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-190 amino acids from human ATG16L.
• UniProt ID: Q676U5
• NCBI: NP_110430.5, NP_942593.2, NP_060444.3, NP_001177196.1, NP_001177195.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Autophagy-related protein 16-1, APG16-like 1, ATG1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot of APG16L (L92) Pab in mouse brain tissue lysate.

Cos7, HEK293, MEF, and Hela cells, left to right, respectively.

Immunofluorescent analysis of U251 cells, using ATG16L Antibody. U251 cells (right) were treated with Chloroquine (50 μM, 16 h). Diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with Autophagy APG16L antibody (L176), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

APG16L Antibody western blot analysis in NCI-H460 cell line lysates (35 ug/lane). This demonstrates the APG16L antibody detected the APG16L protein (arrow).

Anti-ATG16L Antibody at 1:1000 dilution + Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 68 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.