ATF4 Antibody

SKU: orb1253811

Description

ATF4 Antibody

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human
• Application Notes:
  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: ATF4 (Phospho-Ser245) antibody was raised against a peptide sequence around phosphorylation site of serine 245 (N-R-S (p) -L-P) derived from Human ATF4.
• Target: ATF4
• Molecular Weight: 45 kDa
• Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CREB2, TXREB, CREB-2, TAXREB67, EIF4EL1, EIF4F, C/ATF, C/EBP-related ATF, CREB2

Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using ATF4 (Phospho-Ser245).

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF4 (Phospho-Ser245).

Quick Database Links

Gene Symbol

ATF4

UniProt

P18848

RefSeq (Protein):NP_001666.2