AT-533

SKU: orb1685570

Description

AT-533 is a dual Hsp90 and HSV inhibitor that suppresses tumor growth and angiogenesis by blocking the HIF-1α/VEGF/VEGFR-2 signaling axis and downstream pathways like Erk1/2 and Akt/mTOR. It has demonstrated anti-angiogenic activity in vitro by inhibiting HUVEC migration, invasion, and tube formation, supporting its research application in oncology and virology studies.

Research Area

Cardiovascular Research, Epigenetics & Chromatin, Infectious Disease & Virology, Metabolism Research, Signal Transduction

Key Properties

• CAS Number: 908112-37-8
• MW: 410.51
• Purity: 99.67%
• Formula: C₂₃H₃₀N₄O₃
• SMILES: Cc1nn(c2CC(C)(C)CC(=O)c12)-c1ccc(C(N)=O)c(N[C@H]2CC[C@H](O)CC2)c1
• Target: HIF/HIF Prolyl-Hydroxylase,FAK,Akt,VEGFR,HSP,ERK,NF-κB,HSV
• Solubility: DMSO:60 mg/mL (146.16 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.87 mM)

Bioactivity

• In Vivo:
  AT-533 (10 mg/kg; intraperitoneal injection; once daily for 21 days) inhibited the expression of HIF-1α/VEGF signaling pathway-related proteins in a mouse MDA-MB-231 xenograft model of breast cancer . AT-533 (1, 2, 4 mg/kg; intraperitoneal injection; once daily for 30 days) did not cause death, appetite loss, weight loss, or adverse reactions in subacute toxicity tests of Sprague Dawley rats .
• In Vitro:
  AT-533 (0-1350 nM; 24h or 48h) inhibits 20 ng/mL VEGF-induced tube formation, cell migration, and HUVEC invasion. Additionally, AT-533 (2 μM or 75 μM; 24h) hinders the HIF-1α/VEGF signaling pathway in hypoxia-induced breast cancer cells and inhibits Akt/mTOR/p70S6K, Erk1/2, and FAK phosphorylation. It shows anti-angiogenic activity on the chorionic villus (CAM) model at 10 nM and 50 nM over 48h. Furthermore, AT-533 (0.5 μM; 2 or 4 hours) reduces TNF-α, IL-1β, and IL-6 production induced by HSV-1 in RAW264.7 and BV2 cells [1, 2].

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AT533, AT-533, AT 533, Benzamide,2[(trans4hydroxycyclohexyl)amino]4(4,5,6,7tetrahydro3,6,6trimethyl4oxo1Hindazol1yl), HSV, HSP90

Compound Identifiers

PubChem CID

24820800