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Catalog Number | orb420376 |
---|---|
Category | Antibodies |
Description | ASAP1 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Anti-ASAP1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the c-terminus region of human ASAP1 protein. |
Concentration | 1.04 mg/ml |
Dilution range | ELISA: 1:50,000, WB: 1µg/mL |
Form/Appearance | Liquid (sterile filtered) |
Purity | This affinity purified antibody is directed against human ASAP1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity to human 100%, bovine 93%, and rat and mouse 87% homology for the immunogen sequence. Cross-reactivity from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | Q9ULH1 |
NCBI | NP_001234925.1 |
Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Arf-GAP with SH3 domain, ANK repeat and PH domain- Read more... |
Note | For research use only |
Application notes | Anti-ASAP1 Antibody has been tested for use in ELISA and Western Blot. Expect a band approximately ~125kDa using positive control lysates over-expressing ASAP1, or other specific lysates or tissues. Although not tested, this antibody could be useful in Immunofluorescence or Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. |
Expiration Date | 12 months from date of receipt. |
Garone, Maria Giovanna et al. Proteomics analysis of FUS mutant human motoneurons reveals altered regulation of cytoskeleton and other ALS-linked proteins via 3'UTR binding Sci Rep, 10, 11827 (2020)
ELISA, IF, IHC, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
Human | |
0.32-20 ng/mL | |
0.17 ng/mL |
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