ASAP1 Antibody

SKU: orb420376

Description

ASAP1 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:50,000, WB: 1µg/mL
• Reactivity: Human
• Application Notes:
  Anti-ASAP1 Antibody has been tested for use in ELISA and Western Blot. Expect a band approximately ~125kDa using positive control lysates over-expressing ASAP1, or other specific lysates or tissues. Although not tested, this antibody could be useful in Immunofluorescence or Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-ASAP1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the c-terminus region of human ASAP1 protein.
• Purity: This affinity purified antibody is directed against human ASAP1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity to human 100%, bovine 93%, and rat and mouse 87% homology for the immunogen sequence. Cross-reactivity from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.04 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1, 130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein, ADP-ribosylation factor-directed GTPase-activating protein 1, ARF GTPase-activating protein 1, Development and differentiation-enhancing factor 1, DEF-1, Differentiation-enhancing factor 1, PIP2-dependent ARF1 GAP

Western Blot of Rabbit anti-ASAP1 antibody. Lane 1: ASAP1 transfected U118 lysate. Load: 35 µg per lane. Primary antibody: ASAP1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody (p/n orb347654) at 1:70000 for 30 min at RT. Block: BlockOut (p/n orb348644) overnight at 4°C. Predicted/Observed size: 125 kda for ASAP1.

Quick Database Links

UniProt

Q9ULH1

RefSeq (Protein):NP_001234925.1

Garone, Maria Giovanna et al. Proteomics analysis of FUS mutant human motoneurons reveals altered regulation of cytoskeleton and other ALS-linked proteins via 3'UTR binding Sci Rep, 10, 11827 (2020)

PubMed: 32678235