AS1842856

SKU: orb1300489

Description

AS1842856 is a potent and selective Foxo1 inhibitor with an IC50 of 30 nM, directly binding to and suppressing its transcriptional activity. This compound also inhibits autophagy and is widely used in vitro and in vivo to study metabolic regulation, cellular differentiation, and longevity pathways.

Research Area

Cancer Biology, Cell Biology, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 836620-48-5
• MW: 347.4
• Purity: >98%
• Formula: C₁₈H₂₂FN₃O₃
• SMILES: CCn1cc(C(O)=O)c(=O)c2c(N)c(F)c(NC3CCCCC3)cc12
• Target: Transcription factor
• Solubility: Soluble in DMSO (10 mg/ml with warming)

Bioactivity

• Target IC50:
  FOXO1:33nM
• In Vivo:
  METHODS: To assay antitumor activity in vivo, BAY 2416964 (30 mg/kg) was administered orally to NSG mice bearing B16F10-OVA tumors once daily for seven days. RESULTS: BAY 2416964 inhibited tumor growth.BAY 2416964 increased the frequency of immunostimulatory tumor-infiltrating CD8+ T cells and N cells, and decreased the frequency of immunosuppressive GR1-positive myeloid cells and CD206+M2 macrophages.
• In Vitro:
  METHODS: Human monocytes were treated with BAY 2416964 (1 nM-10 µM), kynurenic acid (200 µM), and LPS (10 ng/mL) for 24 h. TNF-α levels were measured by ELISA. RESULTS: kynurenic acid-induced AhR activation inhibited TNF-α production in LPS-stimulated monocytes, and BAY 2416964 dose-dependently rescued this AhR-mediated inhibition.
• Cell Research:
  Fao cells are serum-starved (1 h) and incubated for 30 min with either insulin or AS1842856 at the indicated concentration. Protein lysates are prepared from cells treated with either insulin or AS1842856, and relative concentration of phosphorylated Foxo1 protein is determined by Western blot analysis. (Only for Reference)

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, Inhibitor, Forkhead box protein O1 (FOXO1), Autophagy, AS 1842856, AS1842856, AS-1842856

Compound Identifiers

PubChem CID

72193864