DCLRE1C Antibody

SKU: orb345299

Description

Artemis antibody

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:10,000 - 1:20,000, IHC: 1:1,000, WB: 1:500 - 1:2,000
• Reactivity: Human
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Reactivity in other immunoassays is unknown. This antibody detects a band of approximately 90 kDa (predicted molecular weight: 78 kDa).

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding an internal region of Human ARTEMIS (DCLRE1C DNA cross-link repair 1C).
• Target: DCLRE1C
• Purity: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. This antibody detects a band of the appropriate size in a number of different cell lines. However, it does not detect a band in cell lines that do not express artemis, such as the cell line CJ (see figure) and in an hTERT line (not shown).
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

goat anti-Artemis Antibody, A-SCID antibody, A SCID protein antibody, protein Artemis antibody, DCLRE1C antibody, DCLRE1C, DNA cross link repair 1C antibody, ASCID, SCIDA, SNM1C

Biorbyt's Affinity Purified anti-Artemis antibody was used at a 1:1000 dilution to detect Artemis by immunohistochemistry in human spleen. Positive staining of T cells and B lymphocytes is observed in thymus, lymph nodes and spleen. Tissue was formalin-fixed and paraffin embedded.

Western blot using Biorbyt's Goat-anti-Artemis was used to detect Artemis. Lane 1: AT7BI cell lysates. Lane 2: MO59K cell lysates. Lane 3: MO59J cell lysates. Lane 4: HeLa cell lysates (p/n orb348668). Lane 5: CJ cell lysates. CJ is an Artemis deficient cell line and so no band is visible. Primary Antibody at 1:500 dilution. Signal was detected using standard techniques.

Quick Database Links

Gene Symbol

DCLRE1C

UniProt

Q96SD1

RefSeq:76496495