ARF4 Antibody

• Catalog Number: orb1539389
• Category: Antibodies
• Description: ARF4 Antibody
• Target: ARF4
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Hamster, Human, Mammal, Monkey, Mouse, Rabbit, Rat, Sheep
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: Peptide with sequence C-SEMTDKLGLQSLRN, from the internal region of the protein sequence according to NP_001651.1.
• Tested applications: ELISA, IHC, IHC-P, WB
• Dilution range: IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (0.3 - 1 µg/ml)
• Application notes: Further information: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 19kD band observed in lysates of cell line HeLa (calculated MW of 20.5kD according to NP_001651.1). Recommended concentration: 0.3-1 ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ARF4, ARF2, ADP-ribosylation factor 2, ADP-ribosyl
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ARF4 antibody (0.5 µg/ml) staining of NIH3T3 (A) and HeLa (B) lysates (35 µg protein in RIPA buffer). Detected by chemiluminescence.