ARF1 Antibody

SKU: orb1247778

Description

ARF1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 18kDa band observed in lysates of cell lines HEK293, A431 and HepG2 (calculated MW of 20.7kDa according to NP_001019397.1; NP_001649.1; NP_001019398.1; NP_001019399.1). Recommended concentration: 1-3µg/mL. Primary incubation was 1 hour.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-EGLDWLSNQLRNQK
• Target: ARF1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ARF1, ARF 1, ADP-ribosylation factor 1, ADP ribosylation factor 1

orb1247778 (1 ug/ml) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

ARF1

UniProt

P84077

RefSeq (Protein):NP_001649.1, NP_001019398.1, NP_001019397.1, NP_001019399.1