Ardisiacrispin A

SKU: orb1303350

Description

Ardisiacrispin A (Deglucocyclamin) is a cytotoxic agent with a GI(50) of 1.56 µM against HepG2 cells, inducing apoptosis and microtubule disassembly in Bel-7402 cells. The Ardisiacrispin (A+B) mixture also inhibits HL-60 cell proliferation by arresting the cell cycle at S phase and triggering apoptosis, highlighting its utility in anticancer research.

Research Area

Infectious Disease & Virology

Key Properties

• CAS Number: 23643-61-0
• MW: 1061.21
• Purity: 99.43% (May vary between batches)
• Formula: C₅₂H₈₄O₂₂
• SMILES: [H][C@@]12C[C@](C)(CC[C@@]11CO[C@@]22CC[C@]3([H])[C@@]4(C)CC[C@H](O[C@]5([H])OC[C@H](O[C@]6([H])O[C@H](CO)[C@@H](O)[C@H](O)[C@H]6O[C@]6([H])OC[C@@H](O)[C@H](O)[C@H]6O)[C@@H](O)[C@H]5O[C@]5([H])O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O)C(C)(C)[C@]4([H])CC[C@@]3(C)[C@]2(C)C[C@H]1O)C=O
• Target: HSV,Antibiotic
• Solubility: DMSO:10.62 mg/mL (10.01 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (1.88 mM)

Bioactivity

• In Vitro:
  Ardisiacrispin A+Ardisiacrispin B is a mixture of Ardisiacrispin A and ardisiacrispin B, derived from Ardisia crenata with a fixed proportion (2:1). METHODS AND RESULTS:The present study was conducted to investigate its anticancer activity on human cancer cells and its underlying mechanism of action. The (IC50)s of Ardisiacrispin A+Ardisiacrispin B on proliferation of several human cancer cell lines were in the range of 0.9-6.5 microg/ml by sulphorhodamine B-based colorimetric assay, in which Bel-7402 was the most sensitive cell line. Moreover, Ardisiacrispin A+Ardisiacrispin B induced dose-dependent apoptosis in Bel-7402 cells at doses of 1-10 microg/ml by flow cytometry, and resulted in the changes of the mitochondrial membrane depolarization, membrane permeability enhancement, and nuclear condensation in a dose-dependent manner through high-content screening analysis. Furthermore, Ardisiacrispin A+Ardisiacrispin B could disassemble microtubule in Bel-7402 cells; the fluorescence intensity of microtubules decreased at the concentration of 5-20 microg/ml. CONCLUSIONS: These findings suggest that Ardisiacrispin A+Ardisiacrispin B could inhibit the proliferation of Bel-7402 cells by inducing apoptosis and disassembling microtubule.
  Read more...

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Deglucocyclamin, Ardisiacrispin A, LTS 4, LTS4, LTS-4, inhibit, Inhibitor, HSV, Saxifragifolin B

Compound Identifiers

PubChem CID

138911402