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ARC Rabbit Polyclonal Antibody

Catalog Number: orb576004

DispatchUsually dispatched within 3-7 working days
$ 600.00
Catalog Numberorb576004
CategoryAntibodies
DescriptionRabbit polyclonal antibody to ARC
TargetARC
ClonalityPolyclonal
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman
Predicted ReactivityBovine, Goat, Guinea pig, Mouse, Rat
Form/AppearanceLiquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration0.5 mg/ml
Buffer/PreservativesLiquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
ImmunogenThe immunogen is a synthetic peptide directed towards the middle region of human ARC
Protein SequenceSynthetic peptide located within the following region: ELDLPQKQGEPLDQFLWRKRDLYQTLYVDADEEEIIQYVVGTLQPKLKRF
UniProt IDQ7LC44
MW45 kDa
Tested applicationsIHC, WB
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative nameshArc, Arg3.1
NoteFor research use only
NCBINP_056008
ARC Rabbit Polyclonal Antibody

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 0.8 ug/ml of the antibody was used in this experiment.

ARC Rabbit Polyclonal Antibody

Sample Tissue: Human Fetal Lung, Antibody Dilution: 1.0 ug/ml.

ARC Rabbit Polyclonal Antibody

Sample Type: 293T, Antibody Dilution: 1.0 ug/ml.

ARC Rabbit Polyclonal Antibody

Sample Type: 721_B, Antibody Dilution: 1.0 ug/ml.

ARC Rabbit Polyclonal Antibody

Sample Type: Hela, Antibody Dilution: 1.0 ug/ml.

ARC Rabbit Polyclonal Antibody

Positive control (+): 293T Cell Lysate (2T), Negative control (-): Human Stomach Tumor (T-ST), Antibody concentration: 1 ug/ml.

ARC Rabbit Polyclonal Antibody

Immunohistochemistry with Adrenal tissue at an antibody concentration of 5 ug/ml using anti-ARC antibody (orb576004).

ARC Rabbit Polyclonal Antibody

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/ml in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

ARC Rabbit Polyclonal Antibody

WB Suggested Anti-ARC Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: Transfected 293T.

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