Arachidonic acid

SKU: orb1303125

Description

Arachidonic acid

Research Area

Metabolism Research

Key Properties

• CAS Number: 506-32-1
• MW: 304.47
• Purity: 99.21%
• Formula: C₂₀H₃₂O₂
• SMILES: CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O
• Target: Endogenous Metabolite
• Solubility: DMSO:262.5 mg/mL (862.15 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:10 mg/mL (32.84 mM)

Bioactivity

• Target IC50:
  L-type Ca2+ channel (cardiac myocytes):8.5 μM|Mg2+-ATPase:90 μM|3H-ouabain:32 μM|NaK-ATPase:75 μM
• In Vivo:
  METHODS: To tes the effect o the inflammatory response, Arachidonic acid (150 mg/kg in 1% CMC Na) was administered orally to C57B-/- mice with high-fat diet (HFD)-induced cardiac injury every two days for eight weeks. Results: Arachidonic acid treatment prevented MD2/TLR4 dimerization, induction of inflammatory factors, and tissue damage through TLR4-mediated inflammatory responses in a high-fat diet obese mouse model.
• In Vitro:
  METHODS: RAW264.7 and PBMC-derived macrophages were treated with Arachidonic acid (40-80 µM) for 12-24 h and cell viability was determined by CCK8 assay. Results: Arachidonic acid significantly inhibited macrophage viability in a dose-dependent manner within 12 h, and more Inhibition was observed between 12 h and 24 h. Arachidonic acid was also shown to inhibit macrophage viability in a dose-dependent manner. METHODS: Human breast cancer cells, MDA-MB-231, were treated with Arachidonic acid (8 µM) for 48 h. Caspase activity was measured using a spectrofluorophotometer. Results: MDA-MB-231 cells stimulated with Arachidonic acid for 48 h showed a significant increase in caspase-3 activity, and Arachidonic acid also induced significant activation of caspase-8 and caspase-9.

Storage & Handling

• Storage: keep away from direct sunlight,store under nitrogen,keep away from moisture,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

arachidonate, Arachidonic acid, inhibit, Immunocytophyt, Immunocytophyte, Inhibitor, Endogenous Metabolite, EndogenousMetabolite, Vevodar

Compound Identifiers

PubChem CID

444899