Arachidonic acid

SKU: orb1303125

Description

Arachidonic acid is an omega-6 essential fatty acid, a key membrane phospholipid component and a vital precursor for eicosanoid signaling molecules. It is widely used in research to study inflammation models, such as paw edema, and to investigate lipid metabolism and cell signaling pathways in both in vitro and in vivo systems.

Research Area

Metabolism Research

Key Properties

• CAS Number: 506-32-1
• MW: 304.47
• Purity: 99.78% (May vary between batches)
• Formula: C₂₀H₃₂O₂
• SMILES: CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O
• Target: Endogenous Metabolite
• Solubility: DMSO:262.5 mg/mL (862.15 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:10 mg/mL (32.84 mM)

Bioactivity

• Target IC50:
  L-type Ca2+ channel (cardiac myocytes):8.5 μM|Mg2+-ATPase:90 μM|3H-ouabain:32 μM|NaK-ATPase:75 μM
• In Vivo:
  METHODS: To test the effect on the inflammatory response, Arachidonic acid (150 mg/kg in 1% CMC Na) was administered orally to C57BL/6 mice with high-fat diet (HFD)-induced cardiac injury every two days for eight weeks. RESULTS: Arachidonic acid treatment prevented MD2/TLR4 dimerization, induction of inflammatory factors, and tissue damage through TLR4-mediated inflammatory responses in a high-fat diet obese mouse model.
• In Vitro:
  METHODS: RAW264.7 and PBMC-derived macrophages were treated with Arachidonic acid (40-80 µM) for 12-24 h and cell viability was determined by CCK8 assay. RESULTS: Arachidonic acid significantly inhibited macrophage viability in a dose-dependent manner within 12 h, and more inhibition was observed between 12 h and 24 h. Arachidonic acid was also shown to inhibit macrophage viability in a dose-dependent manner. METHODS: Human breast cancer cells, MDA-MB-231, were treated with Arachidonic acid (8 µM) for 48 h. Caspase activity was measured using a spectrofluorophotometer. RESULTS: MDA-MB-231 cells stimulated with Arachidonic acid for 48 h showed a significant increase in caspase-3 activity, and Arachidonic acid also induced significant activation of caspase-8 and caspase-9.

Storage & Handling

• Storage: keep away from direct sunlight,store under nitrogen,keep away from moisture,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

arachidonate, Arachidonic acid, inhibit, Immunocytophyt, Immunocytophyte, Inhibitor, Endogenous Metabolite, EndogenousMetabolite, Vevodar

Compound Identifiers

PubChem CID

444899