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Catalog Number | orb345282 |
---|---|
Category | Antibodies |
Description | Apolipoprotein E antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, IP, WB |
Reactivity | Human, Rat |
Isotype | IgG |
Immunogen | Apolipoprotein Type E was isolated from human plasma by density gradient centrifugation followed by HPLC purification. |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:4,000 - 1:8,000, IHC: 1:50 - 1:200, IP: 1:100, WB: 1:500 - 1:2,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-Apolipoprotein E has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1% cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein E and has negligible cross-reactivity with Type A-1 A-II, B, C-I, C-II, C-III and J apoLipoproteins. Specific cross-reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross-reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible. |
Conjugation | Unconjugated |
UniProt ID | P02649 |
NCBI | NP_000032 |
Storage | Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | goat anti-Apolipoprotein E3 antibody, Alzheimer di Read more... |
Note | For research use only |
Application notes | Apolipoprotein E antibodies have been tested by western blot and used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. |
Expiration Date | 12 months from date of receipt. |
IHC-P, WB | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Porcine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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