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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | WB: 0.736111111 |
| Reactivity | Human, Rat |
| Predicted Reactivity | Bovine, Canine, Gallus, Mouse, Zebrafish |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser260 of rat tryptophan hydroxylase (TPH), conjugated to keyhole limpet hemocyanin (KLH). |
| Target | Tryptophan Hydroxylase (Ser260) |
| Molecular Weight | 55 |
| Purification | Antigen Affinity Purified from Pooled Serum |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml rAlbumin and 50% glycerol. |
| Concentration | Lot Dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Western blot of rat brainstem lysate showing specific immunolabeling of the ~55 kDa TPH protein phosphorylated at Ser260 in lane one (-). Phosphospecificity is shown in the second lane (P) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (NP), shown in the third lane.
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UniProt Details
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Protocol Information
Tryptophan Hydroxylase (Ser260) Antibody (orb319698)
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