Phospho-TPH (Ser260) Rabbit Polyclonal Antibody

SKU: orb7107

Description

Phospho-TPH (Ser260) Rabbit Polyclonal Antibody

Research Area

Amino Acid Metabolism, Cancer Research, Cell Biology, Energy Metabolism, Hormone Biosynthesis

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human TPH around the phosphorylation site of Ser260 HS(p-S)DP
• Target: TPH1
• Molecular Weight: 49 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Tph1(phospho S260); TPH(phospho Ser260); p-TPH(Ser260); MGC119994; TPH 1; TPH; TPH1; TPRH; Tryptophan 5 hydroxylase 1; Tryptophan 5 monooxygenase 1; Tryptophan 5 monooxygenase; Tryptophan hydroxylase 1; Tryptophan 5-hydroxylase 1; TPH1_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TPH (Ser260)) Polyclonal Antibody, Unconjugated (orb7107) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: HepG2 Cell (Human) Lysate at 40 ug, Primary: Anti-phospho-TPH (Ser260) (orb7107) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 49 kD, Observed band size: 49 kD.

Sample: Lane 1: Mouse Cerebellum tissue lysates, Lane 2: Rat Cerebellum tissue lysates, Primary: Anti-phospho-TPH (Ser260) (orb7107) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 49 kDa, Observed band size: 50 kDa.

Quick Database Links

Gene Symbol

TPH1

UniProt

P17752