Anti-p54nrb Antibody

• Catalog Number: orb340882
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NONO
• Target: NONO
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant full length protein of human p54nrb
• UniProt ID: Q99K48, Q5FVM4, Q15233
• Tested applications: IH, IP, WB
• Dilution range: WB: 1:500-1:2000, IHC-P: 1:50-1:200, IF/ICC: 1:50-1:200, IP: 1:20-1:50
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NRB54; Non-POU domain-containing octamer-binding p
• Note: For research use only
• Entrez: 53610, 4841, 317259

Western blot analysis of p54nrb expression in Hela (A), SW620 (B), mouse brain (C), mouse kidney (D) whole cell lysates. (Predicted band size: 43; 54 kD; Observed band size: 58 kD)

Immunohistochemical analysis of p54nrb staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.