p54nrb Rabbit Polyclonal Antibody

SKU: orb340882

Description

The p54nrb Antibody is suitable for IHC, IP, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human p54nrb .Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Cancer, DNA Damage, Epigenetics, Immunology

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB: 1:500-1:2000, IHC-P: 1:50-1:200, IF/ICC: 1:50-1:200, IP: 1:20-1:50
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human p54nrb
• Target: NONO
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NRB54; Non-POU domain-containing octamer-binding protein; NonO protein; 54 kDa nuclear RNA- and DNA-binding protein; 55 kDa nuclear protein; DNA-binding p52/p100 complex, 52 kDa subunit; NMT55; p54(nrb); p54nrb

Western blot analysis of p54nrb expression in Hela (A), SW620 (B), mouse brain (C), mouse kidney (D) whole cell lysates. (Predicted band size: 43; 54 kD; Observed band size: 58 kD)

Immunohistochemical analysis of p54nrb staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

NONO

UniProt

Q15233, Q99K48, Q5FVM4

NCBI Gene ID

317259, 53610, 4841