p53 Antibody (Biotin)

• Catalog Number: orb2653067
• Category: Antibodies
• Description: The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.
• Target: p53
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Conjugation: Biotin
• Reactivity: Human, Primate
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Immunogen: Bacterially expressed full-length wild-type p53
• UniProt ID: P04637
• Tested applications: ELISA, FC, ICC, IHC-P, IP, WB
• Application notes: Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
• Clone Number: BP53-12
• Storage: Store at 2-8°C. Do not freeze.
• Alternative names: BCC7, TRP53, TP53, LFS1
• Note: For research use only
• Entrez: 7157
• Expiration Date: 12 months from date of receipt.

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-p53 (BP53-12) Biotin antibody (concentration in sample 1 μg/ml, Streptavidin APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-p53 (BP53-12) Biotin antibody (concentration in sample 1 μg/ml, Streptavidin APC).