Anti-Omalizumab Neutralizing Antibody ELISA kit

SKU: orb2975632

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Omalizumab has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled Human IgE and then pipetted into the wells. Anti-Omalizumab Neutralizing Antibody in the sample competitively binds to Omalizumab with the biotin-labeled Human IgE. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Anti-Omalizumab Neutralizing Antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.

Key Properties

• Sample Types: Plasma, Serum
• Assay Type: Quantitative
• Range: 156.25 - 10,000 ng/mL
• Sensitivity: 82.6 ng/mL

Storage & Handling

• Storage: When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20°C, the rest reagents should be store at 4°C.
• Disclaimer: For research use only

Alternative Names

IGE25,olizumab,pSVIE26,rhuMab-E25, CAS: 242138-07-4