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Catalog Number | orb2608858 |
---|---|
Category | Antibodies |
Description | Anti-Nucleolin/NCL Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | Rabbit IgG |
Conjugation | PE |
Reactivity | Human, Monkey, Mouse, Rat |
Form/Appearance | Lyophilized |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Purification | Immunogen affinity purified. |
Immunogen | E.coli-derived human Nucleolin/NCL recombinant protein (Position: K219-A629). |
UniProt ID | P19338 |
MW | 100-110 kDa |
Tested applications | ELISA, FC, ICC, IF, IHC, WB |
Application notes | Western blot, 0.25-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells ELISA, 0.1-0.5μg/ml. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
Cross Reactivity | No cross-reactivity with other proteins. |
Antibody Type | Primary Antibody |
Storage | Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Nucleolin; Protein C23; NCL |
Note | For research use only |
ELISA, FC, ICC, IF, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, IP, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |