Anti-LAG-3 [C9B7W]

SKU: orb669763

Description

Mouse monoclonal antibody to Lag3

Research Area

Epigenetics, Immunology

Images & Validation

• Tested Applications: Blocking, FC, IP
• Reactivity: Mouse

Key Properties

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: C9B7W
• Immunogen: This antibody was raised by immunising Lewis rats with a murine LAG-3:Ig fusion protein.
• Target: LAG-3
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

CD223; LAG3; Lymphocyte activation gene 3 protein

Binding curve of anti-Lag3 antibody C9B7W (orb669762) to recombinant mouse Lag3 Fc-Fusion Protein. ELISA Plate coated with recombinant mouse Lag3 Fc-Fusion Protein at a concentration of 2 µg/ml. A 4-fold serial dilution from 5000 ng/ml was performed using orb669762. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.

Immunofluorescence staining of mouse splenocytes using anti-Lag3 antibody C9B7W. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of C9B7W (orb669762) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb669762, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (orb256390) followed by Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

LAG-3

UniProt

Q61790