Anti-LAG-3 [C9B7W]

• Catalog Number: orb669762
• Category: Antibodies
• Description: Mouse monoclonal antibody to Lag3
• Target: LAG-3
• Clonality: Monoclonal
• Species/Host: Rat
• Isotype: Rat IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: This antibody was raised by immunising Lewis rats with a murine LAG-3:Ig fusion protein.
• UniProt ID: Q61790
• Tested applications: Blocking, FC, IP
• Clone Number: C9B7W
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD223; LAG3; Lymphocyte activation gene 3 protein
• Note: For research use only

Binding curve of anti-Lag3 antibody C9B7W (orb669762) to recombinant mouse Lag3 Fc-Fusion Protein. ELISA Plate coated with recombinant mouse Lag3 Fc-Fusion Protein at a concentration of 2 µg/ml. A 4-fold serial dilution from 5000 ng/ml was performed using orb669762. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.

Immunofluorescence staining of mouse splenocytes using anti-Lag3 antibody C9B7W. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of C9B7W (orb669762) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb669762, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (orb256390) followed by Alexa Fluor® 488 secondary antibody.