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Anti-Hu CD222 Purified

Catalog Number: orb44197

DispatchUsually dispatched within 5-10 working days.
$ 290.00
Catalog Numberorb44197
CategoryAntibodies
DescriptionMouse Monoclonal to CD222.
ClonalityMonoclonal
Clone NumberMEM-238
Tested applicationsFC, IP, WB
ReactivityHuman, Primate
IsotypeMouse IgG1
ImmunogenRecombinant Vaccinia virus encoding CD222.
Antibody TypePrimary Antibody
Concentration1 mg/ml
Dilution rangeFlow cytometry: Extracellular and intracellular staining. Recommended dilution: 2-6 μg/ml. Western blotting: Non-reducing conditions.
PurityPurified by protein-A affinity chromatography.
ConjugationUnconjugated
TargetCD222
Entrez3482
UniProt IDP11717
RRIDAB_10993907
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Alternative namesAnti-CD222 antibody, anti-IGF2 receptor antibody,
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NoteFor research use only
Application notesFlow cytometry: Extracellular and intracellular staining. Recommended dilution: 2-6 μg/ml. Western blotting: Non-reducing conditions. Recommended dilution: 1-2 µg/ml.
Expiration Date12 months from date of receipt.
Anti-Hu CD222 Purified

Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD222 (MEM-238) purified antibody (concentration in sample 2 µg/ml) GAM APC.

Anti-Hu CD222 Purified

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD222 (MEM-238) purified antibody (concentration in sample 2 µg/ml) GAM APC.

Anti-Hu CD222 Purified

Anti-Hu CD222 Purified (clone MEM-238) works in WB application under non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, K562, Raji, and HeLa cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 7% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody MEM-238 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Mouse anti-GAPDH monoclonal antibody FF26A conjugated with DyLight 680 (0.1 µg/ml), was used as the loading control (red). Multiplex fluorescent Western blot detection was performed. CD222 molecules were detected at ~250 kDa in all analysed cell lines.

  • Anti-Hu CD222 Purified [orb402817]

    FC,  IP,  WB

    Human

    Monoclonal

    Unconjugated

    0.1 mg