Anti-HLA-DR1 (empty) Purified

• Catalog Number: orb44258
• Category: Antibodies
• Description: Mouse Monoclonal to HLA-DR1.
• Clonality: Monoclonal
• Clone Number: MEM-267
• Tested applications: ELISA, FC, WB
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: Purified, insoluble DR1 beta chain (DRB1*0101) expressed in E. coli inclusion bodies.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: The antibody MEM-267 stains immature dendritic cells that express empty cell surface MHC molecules, but not cells that express predominantly peptide loaded forms. Western blotting: Recommended dilution: 1 µg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: HLA-DR1 (empty)
• RRID: AB_10994893
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-HLA-DR1 (empty) antibody
• Note: For research use only
• Application notes: Flow cytometry: The antibody MEM-267 stains immature dendritic cells that express empty cell surface MHC molecules, but not cells that express predominantly peptide loaded forms.Western blotting: Recommended dilution: 1 µg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of human HLA-DR1 positive lymphocytes (red-filled) from HLA-DR1 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human HLA-DR1 (empty) (MEM-267) purified antibody (concentration in sample 9 µg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood using anti-human HLA-DR1 (empty) (MEM-267) purified antibody (concentration in sample 9 µg/ml, GAM APC).

Anti-HLA-DR1 (empty) (clone MEM-267) works in WB application under reducing and non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Raji and Jurkat cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 10% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with mouse IgG2b monoclonal antibody MEM-267 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed. HLA-DR1 molecules were detected at ~25 kDa in Raji cell line.