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Anti-Histone H3 Antibody

Catalog Number: orb338856

DispatchUsually dispatched within 5-10 working days
$ 170.00
Catalog Numberorb338856
CategoryAntibodies
DescriptionRabbit polyclonal antibody to HIST1H3A
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IH, WB
ReactivityHuman, Mouse, Porcine, Rat, Zebrafish
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H3. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500, FACS: 1:100-200
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetHIST1H3A
Entrez100361558, 319152, 8353, 8968, 8351, 8357, 8350, 15078, 15077, 291159, 8352, 8355, 8356, 8354, 8358
UniProt IDP84245, P68433, P84244, Q71DI3, P68431, P84243, P84228, Q6LED0
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti HIST1H3A antibody, anti H3FA antibody, anti H
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-Histone H3 Antibody

Western blot analysis of Histone H3 expression in MCF7 (A), Raw264.7 (B), mouse heart (C), rat heart (D) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 17 kD)

Anti-Histone H3 Antibody

Immunohistochemical analysis of Histone H3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Histone H3 Antibody

Immunofluorescent analysis of Histone H3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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