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GRB2 Antibody
Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit polyclonal |
| Clone No. | PAb (554) |
| Immunogen | Synthetic peptide corresponding to C-terminus of human Grb2 (amino acids 198-217). |
| Target | GRB2 |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Western bloting analysis of human GRB2 using rabbit polyclonal antibody PAb (554) on lysates of MOLT-4 and RAMOS cell lines under reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of rabbit anti-GRB2 antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. A specific band was detected for GRB2 at approximately 24 kDa.
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GRB2 Antibody (orb44786)
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