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| Catalog Number | orb18345 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to GRB2 |
| Target | GRB2 |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Porcine, Rat |
| Predicted Reactivity | Bovine, Canine |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Protein Sequence | PRNYVTPVNRNV |
| RRID | AB_10755386 |
| MW | 25.2; 20.6 |
| Tested applications | ELISA, IHC, WB |
| Dilution range | ELISA: 1:32000, WB: 0.05-0.15 μg/ml, IHC-P: 1-2 μg/ml |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Tonsil, stains mantle zone and to a lessser extent germinal centre. Recommended concentration, 0.1-0.3ug/ml.WB: Approx 30kDa band seen in Jurkat lysate. Recommended for use at 1-3 μg/ml. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti GRB2 antibody, anti growth factor receptor-bo Read more... |
| Research Area | Cancer, Epigenetics, Infectious Diseases |
| Note | For research use only |
| Entrez | 2885 |

Western blot analysis of Jurkat lysate using GRB2 antibody

Immunohistochemical staining of Human Tonsile using GRB2 antibody

Western blot analysis of Mouse and Rat Brain and Spleen lysates using GRB2 antibody

Western blot analysis of Human Thymus (Lane 1) and Molt4 (Lane 2) lysates using GRB2 antibody

Immunohistochemical staining of Human Lymph Node using GRB2 antibody

Immunohistochemical staining of Human Lymph Node using GRB2 antibody

Western blot analysis of Mouse and Rat Brain and Spleen lysates using GRB2 antibody

Western blot analysis of Human and Pig Spleen lysates using GRB2 antibody
WB | |
Bovine, Canine, Equine, Guinea pig, Rabbit, Rat, Zebrafish | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
Human | |
0.16-10 ng/mL | |
0.063 ng/mL |
Human | |
0.16-10 ng/mL | |
0.057 ng/mL |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
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