GFP Antibody

• Catalog Number: orb44764
• Category: Antibodies
• Description: Rabbit Polyclonal to GFP.
• Target: GFP
• Clonality: Polyclonal
• Isotype: Rabbit polyclonal
• Conjugation: Unconjugated
• Reactivity: All
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified by ligand affinity chromatography.
• Immunogen: EGFP, a native full-length protein
• UniProt ID: P42212
• RRID: AB_10995964
• Tested applications: ICC, IP, WB
• Application notes: Western blotting: Recommended dilution 0.5-1.5 μg/ml; positive control: transfected cells; negative control: non-transfected cells. Immunocytochemistry: Recommended dilution 1-3 μg/ml.
• Antibody Type: Primary Antibody
• Clone Number: PAb (476)
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Green fluorescent protein, EGFP, EYFP
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunoprecipitation of GFP-NLS from HEK293 cells using anti-GFP antibody. HEK293 cells were transfected with expression construct encoding GFP-NLS protein. Twenty hours post transfection cells were lysed in non-denaturating conditions (Lysis buffer: 20 mM Tris, pH 7.5, 100 mM NaCl, 0.5% Triton X-100, inhibitors of proteases). Aliquots of cell lysate were immunoprecipitated using a polyclonal anti-GFP antibody (lane 2) or a pre-immune rabbit serum (lane 3). Immunoprecipitates together with a sample of the cell lysate (lane 1) were separated on SDS-PAGE polyacrylamide gel and immunoblotted with the anti-GFP antibody. The positions of molecular weight markers in kDa are indicated at the left.

Immunocytochemistry staining (confocal microscopy) of COS-7 cells transfected with expression constructs encoding membrane-tethered EGFP (membrane-EGFP; top) or nuclear Polycomb 2-EYFP fusion protein (Pc2-EYFP; bottom). The natural fluorescence of the produced proteins is shown in the green channel (left), polyclonal anti-GFP antibody signal was detected in the red channel (right). The system was carefully tested for overlap of these two optical channels and images were scanned separately in sequential scanning mode. The blue nuclear stain is also shown.