Anti-Fibrillarin (Mouse Monoclonal)

SKU: orb1140453

Description

Mouse monoclonal antibody to Fibrillarin

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution Range: For western blots of yeast protein samples: 1:1,000 (cell lysates) to 1:5,000 (nuclear fractions), followed by chemiluminescent detection (ECL). For other (non-ECL) western detection methods: 1:500 to 1:2,500. To detect mammalian fibrillarin on western blots by ECL: 1:250. For immunofluorescence on yeast cells: 1:500 to 1:2,500. For IF of mammalian cells: 1:250.
• Reactivity: Other
• Application Notes:
  For western blots of yeast protein samples: 1:1,000 (cell lysates) to 1:5,000 (nuclear fractions), followed by chemiluminescent detection (ECL). For other (non-ECL) western detection methods: 1:500 to 1:2,500. To detect mammalian fibrillarin on western blots by ECL: 1:250. For immunofluorescence on yeast cells: 1:500 to 1:2,500. For IF of mammalian cells: 1:250.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Clone No.: B50H5
• Immunogen: Raised against yeast fibrillarin, a highly conserved nucleolar protein present in small nucleolar ribonucleoprotein (snoRNP) complexes.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 100ug/100ul
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only