EPHA2 Antibody

SKU: orb1174003

Description

Anti-EPHA2 antibody(BFO389), Rabbit mAb

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, FC, WB
• Dilution Range: ELISA 1/5000-10000|Flow Cyt 1/100
• Reactivity: Human

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: BFO389
• Target: EPHA2
• Purification: Purified from cell culture supernatant by affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ARCC2; CTPA; CTPP1; CTRCT6; ECK

EPHA2 protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-EPHA2 on Expi293 cells transfected with human EPHA2 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).

Flow cytometry analysis of antigen binding of rabbit anti-human EPHA2 mAb (orb1174003). (A) orb1174003 does not bind to Jurkat cells that do not express EPHA2. (B) A clear peak shift of orb1174003 was seen compared to the control when incubated with EPHA2-expressing Hela cells, indicating strong binding of orb1174003 to EPHA2. Antibodies were incubated at 5 μg/mL.

Anti-EPHA2 antibody (SKU# orb1174003) at 1/1000 dilution.Lane: Hela, whole cell lysate.Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution.Predicted band size: 108 kDa.Observed band size: 110 kDa

Quick Database Links

Gene Symbol

EPHA2

UniProt

P29317