Anti-EPHA2 antibody(DM167), Rabbit mAb

• Catalog Number: orb1174003
• Category: Antibodies
• Description: Anti-EPHA2 antibody(DM167), Rabbit mAb
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: DM167
• Tested applications: ELISA, FC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Recombinant Antibody
• Dilution range: ELISA 1/5000-10000|Flow Cyt 1/100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: EPHA2
• UniProt ID: P29317
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ARCC2; CTPA; CTPP1; CTRCT6; ECK
• Note: For research use only
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 12 months from date of receipt.

EPHA2 protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-EPHA2 on Expi293 cells transfected with human EPHA2 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).

Flow cytometry analysis of antigen binding of rabbit anti-human EPHA2 mAb (orb1174003). (A) orb1174003 does not bind to Jurkat cells that do not express EPHA2. (B) A clear peak shift of orb1174003 was seen compared to the control when incubated with EPHA2-expressing Hela cells, indicating strong binding of orb1174003 to EPHA2. Antibodies were incubated at 5 μg/mL.

Anti-EPHA2 antibody (SKU# orb1174003) at 1/1000 dilution.Lane: Hela, whole cell lysate.Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution.Predicted band size: 108 kDa.Observed band size: 110 kDa