Anti-Human Ephrin Type A receptor 2 [1C1]

• Catalog Number: orb348860
• Category: Antibodies
• Description: Human monoclonal antibody to Ephrin Type A receptor 2
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: 1C1
• Tested applications: ELISA, FC, IF, WB
• Reactivity: Human, Mouse
• Isotype: IgG1
• Immunogen: 1C1 was isolated after screening of a non-immunized human Fab phage library. Selection was based on the ability to bind soluble human recombinant EphA2 (residues 183-510). The Fab was subsequently converted to full length human IgG1.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: Human Ephrin Type A receptor 2
• UniProt ID: P29317
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: EphA2; Epithelial cell kinase; Tyrosine-protein ki
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Peng, Li et al. Structural and functional characterization of an agonistic anti-human EphA2 monoclonal antibody J Mol Biol, 413, 390-405 (2011)

PubMed: 21867711

Immunofluorescence staining of fixed LL/2 cells with anti-Human Ephrin Type A receptor 2 antibody 1C1 Fixed murine Lewis Lung cells were stained with the chimeric mouse IgG1 version of 1C1 at 2 µg/ml and a donkey anti-mouse IgG-AF488 (20 µg/ml) for 90 mins. Nuclei stained with Hoechst. Cells captured at 20x magnification. Panel A shows a merge of the fluorescence channels, whilst panel B shows the DIC image. In this fixed sample the cell membrane is clearly outlined.

Immunofluorescence staining of live LL/2 cells with anti-Human Ephrin Type A receptor 2 antibody 1C1 Live murine Lewis Lung cells were stained with the chimeric mouse IgG1 version of 1C1 at 2 µg/ml and a donkey anti-mouse IgG-AF488 (20 µg/ml) for 90 mins. Nuclei stained with Hoechst. Cells captured at 20x magnification. Panel A shows the AF488 signal, panel B the DIC image and panel C a merge of fluorescence (AF488 + Hoechst) with the DIC image. In this live sample, antibody internalisation is observed.