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Catalog Number | orb340887 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to CPT1A |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | Recombinant full length protein of human CPT1A |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:500-1:2000, IF/ICC: 1:50-1:200 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | CPT1A |
Entrez | 25757, 12894, 1374 |
UniProt ID | P50416, P32198, P97742 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | CPT1; Carnitine O-palmitoyltransferase 1, liver is Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of CPT1A expression in HL60 (A), THP1 (B), BT474 (C) whole cell lysates. (Predicted band size: 86; 88 kD; Observed band size: 88 kD)
Immunofluorescent analysis of CPT1A staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA | |
Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
iFluor647 |