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Anti-BHMT Antibody

Catalog Number: orb666135

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb666135
CategoryAntibodies
DescriptionRabbit polyclonal antibody to BHMT
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, WB
ReactivityHuman, Mouse, Rat
ImmunogenRecombinant fusion protein of human BHMT. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500-2000, IF: 1-50-200
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetBHMT
Entrez81508, 635, 12116
UniProt IDQ93088, O35490, O09171
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesAnti-Betaine--homocysteine S-methyltransferase 1 a
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-BHMT Antibody

Western blot analysis of BHMT expression in HL60 (A), rat kidney (B) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 45 kD)

Anti-BHMT Antibody

Immunofluorescent analysis of BHMT staining in human liver cancer tissues. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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    ELISA,  FC,  ICC,  IF,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
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    ELISA,  IHC,  WB

    Canine, Human, Mouse, Rat

    Goat

    Polyclonal

    Unconjugated

    100 μg
  • Anti-BHMT Antibody [orb692162]

    ELISA,  FC,  WB

    Human, Monkey, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μg, 10 μg
  • Anti-BHMT Rabbit Monoclonal Antibody [orb866394]

    IHC,  IP,  WB

    Human

    Rabbit

    Monoclonal

    Unconjugated

  • Anti-BHMT Antibody [orb2598588]

    ELISA,  FC,  ICC,  IF,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    iFluor647

    100 μg