Ankaflavin

SKU: orb1220225

Description

Ankaflavin, isolated from Monascus-Fermented red rice, is a PPARγ agonist with anti-inlfammatory activity. Ankaflavin exhibits selective cytotoxic effect and induces cell death on cancer cells.

Key Properties

• CAS Number: 50980-32-0
• MW: 386.48
• Purity: >98% (HPLC)
• Formula: C??H??O?
• SMILES: O=C([C@@H]1C(CCCCCCC)=O)O[C@]2(C)[C@]1([H])CC(C=C(/C=C/C)OC3)=C3C2=O
• Target: PPAR

Bioactivity

• In Vivo:
  Ankaflavin (10 mg/kg; p.o. ; daily for 28 days) shows antidiabetic and anti-inflammatory activity, improves liver function and pancreatic function. Animal model: Wistar rats (4 weeks of age), diabetes was induced by treating them with Methylglyoxal (MG) (600 mg/kg; oral) for 4 weeks. Dosage: 10 mg/kg. Administration: Oral administration for 28 days. Result: Exerted PPARγ agonist activity. Effectively reduced AGE (advanced glycation end-products) levels in serum, liver, and pancreas of MG-induced rats.
• In Vitro:
  Ankaflavin (0-50 μg/mL, 48 h) shows cytotoxicity against cancer cells with no significant toxicity toward normal cells. Ankaflavin (0-30 μg/mL, 0-48 h) arrests Hep G2 cell cycle at sub-G1 phase in a dose- and time-dependent manner. Ankaflavin (25 μg/mL, 48 h) induces Hep G2 cell apoptosis. Cell Cytotoxicity Assay Cell line: A549, Hep G2, MRC-5 and WI-38. Concentration: 1, 10, 25, and 50 μg/mL. Incubation time: 48 h. Result: Showed cytotoxicity against A549 and Hep G2 cells in a dose-dependent manner with no significant toxicity toward normal cells (MRC-5 and WI-38). Cell Cycle Analysis Cell line: Hep G2 cells. Concentration: 15, 20, 25, and 30 μg/mL. Incubation time: 12, 24, 36, and 48 h. Result: Induced a distinct sub-G1 peak in Hep G2 cells in a dose- and time-dependent manner. Apoptosis Analysis Cell line: Hep G2 cells. Concentration: 25 μg/mL. Incubation time: 48 h. Result: Exhibited significant chromatin condensation (fluorescent spot) through Hoechst staining.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

PPAR